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In this research we will examine how variations in the nitrate-to-phosphate ratio of the growth medium affect the particulate N:P in batch cultures of Skeletonema costatum at irradiances that limit and saturate growth rate. Batch cultures have the advantage of mimicking the onset of nutrient limitation, while the cells are in a transient state. The main focus is the extent to which the elemental stoichiometry of phytoplankton, at various growth stages reflects the nutrient elemental composition of the culture medium.
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